Dear Arun,

Please go to my publication page and check the following article. Full text is available and we did 2D gel brain proteome in zebrafish but can help for the method. If you want detail method pl. check cross ref. s also or send me a message.

Chronic Unpredictable Stress (CUS)-Induced Anxiety and Related Mood Disorders in a Zebrafish Model: Altered Brain Proteome Profile Implicates Mitochondrial Dysfunction

Chakravarty S et al , 2013

You might try this reference as a starting point.

http://www.mcponline.org/content/early/2010/12/22/mcp.M110.004630

Although I did not see testis specifically, the following are very thorough catalogs of protein analyses, including lots of 2D gel identifications:

http://world-2dpage.expasy.org/swiss-2dpage/

http://www.proteinatlas.org/

Hi Craig A Gelfand

Thank you so much for your reply. Hope it helps for me.

Hi Sumana Chakravarty

Thank you so much for the information i would go through you publications and if i have any doubts would contact you back. Thanks