I am struggling with stock solution of ABTS. I am wondering how to prepare 7 mM of ABTS and 2.4 mM of potassium persulfate?
https://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/mass-molarity-calculator.html
this is a great tool for calculating Mass Molarity.
ABTS 7mM = 192mg in 50ml
Potassium Persulfate 2.4mM = 32mg in 50ml
You can follow the manuscript to prepare a stable ABTS stock solution. I believe it will help you.
You can dissolve 13.2 mg of potassium persulfate in 10 ml of distilled water, call in solution A. Weigh 32 mg of ABTS and dissolve it in 4 ml of distilled water, that is solution B. Combine 4 ml each of solution A and B, mix well, store in a dark room for 16hr. The concentration of ABTS should be 7mM and 2.5 mM of potassium persulfate.
What about the actual test how i can use the mixture to assess antioxidant activity?
and How i can prepare trolox as a standard?
and dilution
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