I am trying to grow human cardiomyocytes from primary cell culture for the first time and wanted to check how ImageJ worked for confluency. However, I don't have access to phase contrast microscope so need to make do with a compound microscope for now. Anyone has any suggestions for plugins / add ons or if there is any other alternate for counting confluency which isn't manual (to keep results consistent enough?)
A compound scope will work just fine for this kind of analysis, as long as it's an inverted scope (objective below the plate). You can adjust the condenser and aperture to increase the contrast between the cells and the plate when you take the images.
You should take a look at this other thread on ResearchGate that does a good job of describing what it sounds like you are trying to do:
https://www.researchgate.net/post/Has-anybody-an-idea-how-to-evaluate-confluency
Hi Aarohi Shah. ImageJ works with any digital image, so images acquired from any microscope can be analyzed. If contrast is good, you can threshold your image and use Analyze Particles to measure the area fraction of cells in your images.
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