I am would like to know if there is any optimized expression cassette for penecillin or beta lactam antibiotic genes or vancomycin.
i have gone through many research paper but most of the antibiotics have the cluster gene expression system and but there is no such expression cassette for it to introduce inot plasmid for expression in bacteria or Archaea.
you suggestion would be appriciated in this matter.
Most standard cloning vectors have an ampicillin resistant gene (which is beta-lactamase). So those are nearly universal.
Unless you are performing protein purification for biochemical analysis, you don't require an optimized cassette. Most the naturally found genes for resistance will express adequately to confer resistance. So you just PCR out the genes from any plasmid carrying that resistance marker.
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