Sayed Abdullah

11 Questions 17 Answers 0 Followers

Questions related from Sayed Abdullah

What could be the Acrylamide ratio for about 30-50kda?

We are optimizing SDS-PAGE, i would like to know the best ratio and percentage for the Staking gel and separation gel of Acrylamide. Its like 37.1:1 and 29.1 and 19.1 and also 30% and 40%, So i...

07 December 2023 1,124 6 View

I am looking for Optimized gene expression cassette for any Beta Lactam antibiotic or Vancomycin?

I am would like to know if there is any optimized expression cassette for penecillin or beta lactam antibiotic genes or vancomycin. i have gone through many research paper but most of the...

24 October 2023 7,902 1 View

I am looking for Optimized gene expression cassette for optimized expression of antibacterial agants?

I am currently using pET26b(+) plasmid however, the gene is problematic. and reduce the growth of BL21DE3 cell upon iptg induction. how much yeast is efficent for pYES expression vector.

24 October 2023 8,065 1 View

What could be the in-vitro medium for Artificial Human Gut Microbiome Development?

I was looking into developed a sustainable Human gut microbiome in-vitro. If you have gone through any pubished paper or have any idea of developing gut microbiome in-vitro, will be appriciated...

18 October 2023 1,398 0 View

How to prepare bacteriophage slide for SEM analysis?

Someone recommend me to do SEM analysis as TEM is not available right now, i have M13KO7 helper plasmid which i produce m13 phages and i want to know if there is any simple way to prepare a glass...

11 April 2019 2,212 3 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

15 September 2018 4,492 4 View

How we can use Multi-locus Sequence Typing (MLST) tool for finding our desire genes?

MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...

01 January 1970 1,090 3 View

Which statistic can be apply on prevalence of HBV, HAV, Polio and Adeno viruses among different cities?

I have data of HBV, HAV, Polio and Adeno viruses prevalence among different cities? how can i interpret the results based on Odd ratio, risk factor or Mann whitney test? is there any other...

01 January 1970 9,829 4 View

Can plasmid with f1 ori, insert into m13 phage outside the cell?

I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...

01 January 1970 1,634 4 View

How To analyzed my qPCR data in graphical representation?

I got the ct values for Experimental target gene(35.2ct), control target gene(6.7ct), control House Keeping gene(7.0ct) and experimental housekeeping gene(7.1ct). now i got the fold change value...

01 January 1970 7,560 8 View

Planing PhD in Molecular Microbiology any suggestions?

Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...

01 January 1970 4,956 6 View