How to convert a domestic microwave assisted synthesis for dedicated instrumentation?

Hello, I just dived into the topic of microwave assisted synthesis and found a literature I would like to reproduce. The problem is in the literature, a domestic microwave oven is used which we...

27 January 2021 4,788 4 View

What sensors are typically placed on aircraft, especially experimental?

In addition to sensors such as load cells, prandtl tubes, pitot tubes, which other sensors are put on experimental aircraft

19 November 2020 6,945 3 View

How best can i obtain the 'P' parameter for a PID controller to be used on tiltwing UAV?

For the tiltwing UAV which i am currently designing, i am going to use a PID controller which i will develop in MATLAB. I want to determine the most optimum value for the 'P' parameter...

01 November 2020 7,324 7 View

How do I solve the warning "Excessive distortion at a total of 6 integration points in solid (continuum) elements" (Abaqus)?

Hi, I simulate a nano - 2D indentation process in Abaqus 6.16. The simulation is in Abaqus/Standard and non-linear is switched on. The process is force controlled - I typ in the force and want the...

28 April 2018 925 5 View

Does anyone have experience with the ABI StepOne Real-Time PCR system?

10 September 2014 4,651 4 View

What is the most reliable and affordable technique for transcript profiling eukaryotic plants?

17 July 2014 8,821 2 View

What is virus titer/concentration?

I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus...

19 June 2014 2,170 10 View

Can I use in vitro transcription of cloned cDNA to generate RNA for calibration in a qRT-PCR?

I would like to perform absolute quantification of RNA viruses in plant tissue and was wondering if approaching this through in vitro transcription makes sense.

26 January 2014 6,320 5 View

Is there a possibility to get involved in this project: Neuroeconomics?

I am from South Africa..Masters' student in Economics, also employed at the Department of Economics at University of South Africa. My studies focus on Neuroeconomics. It is a very interesting...

01 January 1970 4,273 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View