I am looking for a protocol for making macrophage stimulating factor (M-CSF) in house?

More Sujatha Manthri's questions See All

Can someone suggest the most reliable database to check to understand the COVID 19 spread both globally and particularly in India?

I would like to map the spread of COVID 19 in specific locations to understand the social and economic parameters that could have caused the spread on the contagion. In this context, the data from...

30 April 2020 5,953 17 View

Euclidean distance -to water, forest and urban areas?

I am trying to model my road-kill data using MAXENT. I want to consider distance to water, forest and urban areas separately for the model. I have.shp file. Pls anybody simply explain me how to...

17 July 2019 1,679 3 View

Does anybody know where can I download land use map for Australia for free?

Pls tell me a source to download the land use map and terrain for Australia for free?

17 July 2019 2,521 4 View

How to prepare a questionnaire to assess the knowledge and attitude of nurses on clozapine medication? Or is their any questionnaire available?

Good morning, I am thinking of doing my masters thesis on assessing the knowledge and attitude of nurses on clozapine medication. I am looking for preparing a questionnaire to assess the...

18 October 2018 9,075 3 View

Why do I get these results for my SDS PAGE analysis of spirulina protein?

how to get a good band on SDS PAGE analysis for spirulina protein. In my results showed dye staining bands on the sample loaded well. The same procedure was follow on BSA sample given clear band...

23 August 2015 2,405 13 View

If a person is comfortable in sharing and happy maintaining a relationship with a person, regardless of gender, why is the world busy chasing them?

We the social beings are very good at conveying, communicating, and maintaining relationships. In that process we often choose a convenient way for the expression of our feelings. In the initial...

06 March 2014 356 3 View

How to constitute biological replications in RT-qPCR ?

In RT-qPCR can specimens/ samples from different plants be pooled to form a biological replication? If samples can be pooled from different individuals/plants, can the biological replications be...

01 January 1970 6,624 9 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Debaki Ranjan Howlader

I have used the L929 cell line which is a adipocyte cell line known for its spontaneous secretion of M-CSF in the culture supernatant. It is a fibroblast, adherent cell line. It needs minimal care and produces huge amount of M-CSF (I did not measure the amount, though). DMEM + 10% FBS is enough for its growth in presence of 5% CO2.