NSG mice are the best mice due to their increased acceptance of xenografts. However, if you just need to see subcutaneous tumor formation and tumor size measurements, nude mice are better option.

Thanks brother, I'm just looking into the subcutaneous tumour formation at the moment... Have you come across the syngenic xenograft models? what are their advantages over subcutaneous nude mice tumours?

Hello Adeel,

NSG, are severely immunocompromised, featuring absence of mature T & B cells, lack of functional NK cells. NSG mice engraft the widest range of normal and cancerous human cells of any immunodeficient strain.They have good tumor take up and growth rate.

> on the other hand, nu/nu nude mice are not completely immunocompromised they are unable to produce T cells but they have B Cells and their precursors.so we can say that Nu/Nu are less efficient tumorogenic than NSC.

> Nu/nu mice are hairless.so animal handling & taking tumor volume with caliper in Nu/nu is easy compare to NSC.

NSC and Nu/nu both have some good and bad features. Now it totally depends upon your expeiment and what are you looking for.

Simultaneously i would like to add my experiences as i have used ovarian carcinoma, SKOV-3 and A-2780 cell lines induced xenograft nude mice models recently. SKOV-3 takes more time to grow upto palpable size than A-2780.but both gave good response and worked well.

Hope it helps you.

Best luck..

milind

Hi Adeel,

We use Nude mice routinely for ovarian models here at iQ Biosciences. NSG or NOD/SCID can work as well but I would ask you to consider what mechanism you may be testing for efficacy. Here is a great link for you to take all those points into consideration: http://jaxmice.jax.org/cancer/xenoHosts.html

Other leading contract research organizations such as Charles River or In-vivo Technologies also use CB17/SCID as well for Ovarian models.

Best Regards,

Omar

We use either athymic balb/c mice or NOD/SCID mice in our ovarian cancer xenografts (http://altogenlabs.com/xenograft-models/ovarian-cancer-xenograft/). You don't want to have the host immune system attacking the tumor as its growing, and preventing regression through immunodeficient mice is the best way to do so.