the extract was only soluble in acetic acid
You may try solvents (Butanol: Acetic Acid: Water) with different percentages or go for reverse phase TLC.
One is BAW solvent system (n-Butanol, Acetic acid and Double distilled water) with different gradients, another is methanol-water-acetic acid solvent system
And try to dissolve the crude extract in methanol or DMSO
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