I am currently doing some CRISPR/Cas9 KO on human kidney cell lines?

11 July 2024 1 3K Report

I did not found any specific article about kidney ACHN cell lines treatment with Puromycin. How much concentration per mL should be suitable?

Jianfeng Lin

you should always do a minimum killing assay to find out the minimum concentration of puromycin to kill all the cells.

From my personal experience, epithelial cells are very sensitive to puromycin, and i used only 0.5ug/ml of puromycin to kill all my epithelial cell lines. For many other cell lines, i have seen 1-10ug/ml of puromycin being used.

I would suggest you set up a preliminary assay to test the 0/5. 1, 2, 4, 6, 10 ug/ml of puromycin to find out the minimum concentraion to use in your cell line.

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