Dear all,
I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice.
The culture conditions are:
-IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S
-Medium replaced each 3-4 days
-When confluent, adherent cells were trypsinized and subcultured (process for MSCs purification)
Shape of cells in general looks quite normal (spindle shaped) but I got curious about some cells with a really weird morphology (please see the picture attached), what are these cells? It seems more cells fused together or phagocyted from a bigger cells.
I thank you in advance for your help.
Best,
Giulia
Thanks Utsa Roy , I hope so! But I am still wondering what is this morphology/why they are like this as it seems that there is a big cells and many inside.
Are you able to see how many nuclei do those very big cells have? If they have multi nuclei, they might be senescent cells. You should get rid of them after some passages. You also could check by doing senescence staining on your cells to see.