I have some experience with staining mice retinal slices with One-step TUNEL In Situ Apoptosis Kit by Elabscience.

I perfused my mice with 10% NBF and post fix tissue for 48hours in the frig, then cryoprotect the tissue with 30% sucrose in PBS till the tissue sink to the bottom. After cryoprotection, I embed my samples with OCT and slice the with a cryotome into 10um slices.

I pretty much follow the manul, 'cept that I added a post-fixation step (10% NBF, 10mins room temperature) on the secions for better adherence onto the glass slides. And I also find that treating my samples with Proteinase K in 37 celcius degree 10 mins is too harsh, so I made it 10 mins room temperature.

This is a good kit which provide almost everything you need, you can give a try!

Good luck there~

Hi Suman Arya,

How will you make the measurements, with flow cytometry or microscopy? Advices will be different for these to different methods. If you plan to use cytometry, strictly follow the instruction in kit insert. After washes, carefully pipet out any fluids, only pellet should stay in tube before you add any reagents, so that you can get clear graphs.

Best wishes

Thank you so much for your valuable suggestions, it would really help me out.