Can anyone suggest me a solvent ratio to increase the gap between these two spots?

After column chromatography I've got these two spots together. And tried a number of solvents ratio to increase the gap between these two spots for further column chromatography but failed.

10 October 2020 1,367 4 View

What is the appropriate length of packed silica for column chromatography?

In the case of column chromatography of plant extract, which is the appropriate length of packed silica of a one-inch diameter and 18 inches long column, and what speed should be used for solvent...

29 September 2020 4,818 8 View

Should I use glass vials for mouse organ preservation for short term or plastic vials are good enough for short term storage?

I have noticed many researchers use both glass and plastic vials for mouse organ preservation. Which one is better in case of short term storage?

19 July 2020 4,928 4 View

Does anyone know which software is available to download that can visualise .svs histology slides for a mac computer?

View histology svs files on a mac, free to download software.

14 July 2020 5,719 1 View

How to measure the distance of chromaticity coordinates and efficiency?

15 June 2017 8,321 3 View

Blackhole MANET OPNET

How to implement a malicious node over OLSR and GRP in MANET to perform black hole attack using OPNET simulator?

30 May 2017 5,758 3 View

Can anyone direct me to the references for the 2-item factor (latent construct)?

I'm looking for references/ published work supporting as well as rejecting a 2-item factor (latent construct). It's well known that 3 item per factor is considered reliable, at minimum.

19 October 2014 2,668 13 View

Has anyone tried to polarize RAW264.7 M1/M2 cells?

Has anyone tried to polarize RAW264.7 murine cell-line (M1/M2-like macrophages) and can share the protocol?

24 April 2014 7,904 4 View

Isolation of low polar compound from hexane fraction of plant extract

What type of solvent ration is appropriate for the isolation of low polar compounds from hexane fraction of plant extract by traditional silica(60-120 mesh) packed column chromatography?

01 January 1970 2,525 8 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View