Hello everyone, I am very new in this regard and learning everyday. I am working on disease resistance in Wheat and want to do QTL mapping. I already have found the chromosome which may have the major resistance(picture is in the attachment), now what could be my next step? I am interested to know about the basic steps but in details. I am using 2 DH population and I have 93 individuals in total and using RQTL software for analysis.
Thank you very much for your kind help.
Article Integrating genome annotation and QTL position to identify c...
Article Q&A: Genetic Analysis of Quantitative Traits
Article A Mixed-Model Quantitative Trait Loci (QTL) Analysis for Mul...
https://www.nature.com/articles/ncomms15452
https://www.rug.nl/research/portal/files/13910115/Complete_dissertation.pdf
Further you have to confirm your identified QTL SNPs by experimental (HRM) using your DNA materials ( resistant and sucestible).
Hi,
In any QTL mapping studies, validation of QTL is very important. May be you need to validate your results in an independent lines or populations. Also, given the size of the wheat genome, I think the population size is quite small and more over they are DH lines. Given these constraints, validation is very critical.
I hope it helps.
Good luck
Sathishkumar Natarajan and Srinivasachary Sri Thank you for your kind help.I will keep asking if I need more sugestions.
1) Narrow down using other set of markers if you can
2) confirm the QTL in other populations/crosses
3) Since we have wheat genome, try to identify genes within that QTL
You just need to validate your QTL in different mapping population and also check if your QTL is the same with other previous publication for disease resistance or not. Good luck!!
Sujan Mamidi Keshav Malla Thank you for your answer.I am trying to use more markers.
Depending upon the disease there are describe reiatance gene in literature. Compare if ur peak co-localize wirh known gene, if yes you can sequnce the parents for allelic polymorphisms. If not, you have to follow positional cloning approach to identify resistant gene..good luck
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