I'm working with Scenedesmus obliquus and Chlorella vulgaris and I'm using hygromycin as a selection antibiotic for a genetic modification. Can you help me on how much hygromycin should I use for the selection?
Thank you very much!, I have tried different concentrations on Scenedesmus and so far 50 ug/mL also seems to be enough for this strain, but i had no data supporting any concentration for Chlorella!, thank you!.
I tested C. Vulgaris, it need more than 60 ug/mL for Hygromycin. However, I am wondering whether I need to put the plate with hygromycin under illumination or not. Can someone answer this question for me? Many thanks