How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate dilution factor based on the highest OD/concentration of sample in ELISA?

I am using Elabscience sandwich ELISA kit (detection range 0.31-20ng/ml), I am using supernatant saliva samples. I am using 4 plates and plotting the standard curve for every plate. In one of the...

27 January 2021 9,759 1 View

Which compounds might cross-react with cortisol in Elisa kit?

Hi, I'm analyzing cortisol in post smolt salmon with two elisa kits. The first is made for human saliva (Fairly cheap) and the second one for fish (three times the price). I was hoping to get...

21 January 2021 6,874 1 View

Recognition of Molecules from a file containing molecular dynamics trajectory?

Can anyone recommend a good code/software/implementation to identify the change of molecules (bond creation/break, number of molecules at a given timestep, etc.)? I am currently working with XYZ...

12 January 2021 1,655 4 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Does anyone know of a reliable source for primary antibodies against oxytocin and vasopressin for IHC studies in rats?

The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.

09 November 2020 833 3 View

Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

Lysozymes extraction from Human saliva?

Need guidance related to isolate it through simple method. Suggest easiest and simplest methods.

17 January 2020 7,347 1 View

Can anyone help me model a receptor protein?

Hi I want to model a receptor protein (Vasopressin 1b) with uniprot id P47901. The third IC loop needs to be handled with more precautions due its long length. I can assure you that the credit...

21 December 2019 4,100 8 View

How to induce an increase in oxytocin and/or vasopressin neurons activity at early-life ?

I would like to know if anyone has a suggestion on how to induce, around postnatal day 10 (P10), an activation of OXT or AVP neurons in mice pups ? A stressor? some handling? a separation/reunion...

11 December 2019 1,344 2 View

DNA Purification-Best Way?

Hello All, Could anyone please suggest me the best way to purify DNA from contaminants such as proteins, RNA, salts and particulates. The DNA was extracted manually from saliva samples therefore...

10 November 2019 3,559 2 View