Analytical results Corrected for Recovery?

Hello, I am working on Quantification of HMF in honey by HPLC-UV Recently i got some test material(honey) I am getting 110% recovery Results should be corrected for recovery? For e.g say i...

02 March 2019 7,732 15 View

How can i write KPI for my position i.e. Lab specialist in Analytical Chemistry Lab?

Please help as i am going to do it for first time. Regards

10 November 2018 9,783 18 View

Uncertainty Measurement?How can i calculate it? What are the different factors which should be considered during calculating UM?

Dear Group members, I want to learn in details about Uncertainty Measurement. Kindly share any presentation or research paper on the same. Regards

08 September 2018 7,606 11 View

Hi, i am working on Validation of Sugars in honey by HPLC- RID detector. I am facing this problem :Retention time shifting with every injection?

RID is highly sensitive to Temperature and mobile phase composition. Column has column oven and even RID detector is constant at 35 degree. Mobile phase is 80% ACN (purged properly) I could not...

08 September 2018 165 20 View

How can i plan Method validation for - Determination of Hmf in honey by HPLC-UV?

06 July 2018 430 19 View

Accuracy: How can i prepare samples for Accuracy/% Recovery Calculations?

Method validation: HMF in honey : HPLC(UV) How can i prepare samples for Accuracy/% Recovery Calculations?Should I spike in water (Blank) or Honey (sample)? Regards

06 July 2018 2,772 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View