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Hello, I am working on Quantification of HMF in honey by HPLC-UV Recently i got some test material(honey) I am getting 110% recovery Results should be corrected for recovery? For e.g say i...
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Please help as i am going to do it for first time. Regards
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RID is highly sensitive to Temperature and mobile phase composition. Column has column oven and even RID detector is constant at 35 degree. Mobile phase is 80% ACN (purged properly) I could not...
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Method validation: HMF in honey : HPLC(UV) How can i prepare samples for Accuracy/% Recovery Calculations?Should I spike in water (Blank) or Honey (sample)? Regards
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My Column is Shimadzu SHIM PACK VP-ODS (4.6 × 150mm x 5 µm) RP and Mobile phase is 10% Methanol Kindly suggest Thanks in advance
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Kindly suggest !
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