How would you explain a decline in the fluorescence observed in a Real-Time reverse transcription PCR when starting from a very diluted RNA sample?

Andy Caballero Méndez @Andy-Caballero-Mendez

11 April 2020 0 10K Report

How would you explain a decline in the fluorescence observed in a Real-Time reverse transcription qPCR when starting from a very diluted RNA sample?

More Andy Caballero Méndez's questions See All

What skills should today's teachers take into account to teach students and prepare them for a long-term future?

Taking into account that each society incorporates into the curricula the necessary knowledge to teach depending on the social demands of each country.

06 June 2024 9,710 14 View

What considerations should be taken when using proprioceptive stimulation in patients with multiple sclerosis?

By working on proprioception, we send stimuli to our brain through multiple pathways (senses, muscle spindles, ligaments, etc.) that will help us improve our balance.

01 June 2024 2,344 3 View

What are the principles to take into account when carrying out techniques with the Kabat Method?

The Kabat Method is a proprioceptive neuromuscular facilitation technique, which uses a series of principles and relaxation or contraction techniques according to the desired objective. This...

01 June 2024 3,107 3 View

How important is the development of research skills in students?

to solve scientific problems in daily professional life

25 May 2024 7,873 11 View

What principle should be taken into account when performing Frenkel exercises?

Frenkel exercises are performed with the intention of recovering coordination of movements.

25 May 2024 7,780 2 View

How important is carrying out a sports detraining program for the health of former athletes?

Sports detraining is necessary for former athletes to be able to physiologically guide the functioning of their body.

25 May 2024 8,404 3 View

Can doping decrease/increase band gaps of semiconducting monolayers in very significant levels?

Hello. I am currently doing computational DFT on a CsGeBr3 (3x3) perovskite monolayer to study its band structure and band gap for my undergraduate thesis, especially with Fe-doping and Biaxial...

17 April 2024 2,438 2 View

Has anyone a protocol or any tip for using the Amicon Ultra for protein concentration?

I am currently expressing a recombinant protein through the transduction of my plasmid into HEK 293T cells. Historically, I've encountered minimal issues and achieved high yields of protein at a...

17 April 2024 9,102 8 View

Error in ABAQUS Restart Model ?

Hi every one I have a model in Abaqus standard with Heat transfer. When I restart this model , Abaqus exit with this error: "Unable to open the file Job-2.mdl" How I can fix this error? Thanks

14 March 2024 2,562 1 View

¿Qué tipo de técnicas podrían usarse para involucrar a la familia de los menores infractores en su reinserción social?

La participación de la familia en la intervención con los menores infractores es indispensable, mediante el trabajo con esta se puede mejorar en gran cantidad las perspectivas de futuro del menor....

10 March 2024 392 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View