Mark O. Pekarsky

9 Questions 9 Answers 0 Followers

Questions related from Mark O. Pekarsky

Is there a "good" transDUCTION protocol?

Hello dear colleagues, I am experiencing strange problems when performing transDUCTION on my CD4/CD8 cells (separated using Miltenyi beads from total PBMCs from a buffy coat). For now on it is...

19 April 2016 9,440 1 View

Any limitations for expression in T-lymphocytes?

Hello everyone, we are trying to transduce T-lymphocytes (TLs) with vector containing IRES, and cannot see any expression using flow cytometry (our target protein is conjugated with GFP). The...

15 March 2016 5,887 3 View

Is it possible to overcome co-expression?

Hello, in my expression cassette I already have 2 proteins that are expressed using IRES. However I'd like to express another protein at the same time (a homing molecule), but, honestly, I do not...

03 February 2016 5,945 3 View

What are best conditions for streptavidin-biotin inactivation?

Hi, We would like to inactivate the activity of biotin added to cell culture, but I am wondering what would be the best way to do it... Normally we have a solution of biotin (powder dissolved in...

11 July 2015 6,373 7 View

Do I need to preculture cell for siRNA experiment?

Hello, I am planning to perform a siRNA knockdown using lifetechnologies Lipofectamine RNAiMAX reagent, so taking into account they require 60-80% confluence of cells on the moment of transfection...

02 March 2015 3,513 6 View

Should we use nanodrop for total protein amount measurements?

Hi, I've been using nanodrop for a long time, however I ised it only for RNA/DNA concentration analysis. In fact, there's a "protein" option in nanodrop that I have never used before, though one...

14 January 2015 9,467 19 View

What are the best conditions for cultivation of Tera-1 (HTB-105) cells?

Hello, colleagues. This is my first time I am trying to grow a cell culture from ATCC. So it is Tera-1 (HTB-105) cells and what they recommend on their site is that the medium has to be the McCoy...

18 November 2014 717 3 View

How would you describe an RNAse free environment - ?

Hello, friends I will need to prepare some total RNA from cells, however I have never did it before. Everyone says that I need to do it in an "RNAse free zone". What that should be in your...

03 November 2014 1,016 3 View

Are there any analogs of ATCC's CRL-7800 cell line?

Hi, friends. Would you be so kind to propose the analog of CRL-7800 cell culture (from ATCC)? We badly need these type of cells to have a hiwi expression in it as a positive control (as described...

28 October 2014 6,714 2 View