Hi Sungira,

I f your purpose is to genotype your samples,  I would suggest you to run your PCR reactions separately for each SSR ,and then co-load them before genotyping with different DYE.

Thank you Amzati

Hi Marvelous,

I guess that you already know the primer sequences of at least 10 markers from other sources. In modern primer developments, the multiplexing is already included in the design. In case of older primer developments, you should look at first at the expected fragment length ranges for each marker and at the annealing temperatures. Within a certain range of fragment lengths, you could combine as much markers as you have free fluorescent dyes. It is much more effective to combine several makers already in the PCR step. We have best experiences with the Qiagen multiplex kits and use up to 9 microsatellite markers in 1 PCR multiplex set.

Good luck

Heike