anybody please help me, how we can differentiate Wolbachia infections into different groups (somewhere Wolbachia infections divided into 8 or 11 or 13 groups), i found Wolbachia in my test specimen but now i don't know which group this infection will fall?
and how we construct phylogenetic relationship between my test specimen and other organisms from different orders
I think the way to classify the Wolbachia strains is to emplify the gene using some certain primers. You can refer to the article in the attachment.
As for the phylogenetic trees, you can get the sequences you need in Gene Bank and construct phylogenetic trees.
Wolbachia is a endobacteria that can be transmitted vertically but also horizontally. So the comparison between different isolates from different Wolbachia hosts is possible. the region of the 16s is to be used for subdivicion groups, with different primers.
Salem, Mr Ali
I advise you use sequencing of the 16S gene is the best methode to identified your groupes.
excuse me, what are the specific specific primers to detect Wolbachia species not only subgroups?
Thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Evolutionary Biology
- Phylogenetics