Ai-Ying Wang

4 Questions 4 Answers 0 Followers

Questions related from Ai-Ying Wang

How to avoid or reduce the impact of bacteria and fungi during extracting wasps' DNA and PCR?

My fig wasps samples were stored in 70% ethanol at 4℃ and utilized for genomic DNA extraction. The genes I used in PCR are 18S, 28S and COI. But some of the sequencing results are bacteria and...

01 January 2018 6,594 3 View

Is there anyone use the software Jane?

Do anyone use or know the software Jane that a tool for the cophylogeny reconstruction problem? I have some problem in importing the .tree format file. What kind of tree I need to import? Thanks!

12 December 2016 507 0 View

What's the structure of 28S rRNA and wingless gene?

I want to reconstruct the phylogeny of wasps. So I have to find some gene and design the primer. Some assays said the 28S rRNA and wingless gene are the good choice. However I don't know the...

06 June 2016 6,670 2 View

Can anyone tell me something about the wingless gene?

I want to use the wingless gene (or wg gene) to reconstruct the phylogenetic tree of wasps. Are there some primers can specifically amplify the wnt-1 gene (450bp) without cloning?

06 June 2016 256 1 View