Poly(ε-caprolactone) (PCL) is a biodegradable polymer that can be used to encapsulate vitamin C for controlled release. One method to quantify vitamin C release from PCL is to use a UV-Vis spectrophotometer.

First, PCL samples containing the encapsulated vitamin C can be prepared. These samples can then be placed in a buffer solution that mimics the environment in which the release is desired to occur. At regular intervals, the buffer solution can be sampled and analyzed using the UV-Vis spectrophotometer.

Vitamin C has a characteristic absorbance peak in the UV-Vis region at a wavelength of 245 nm. By measuring the absorbance of the buffer solution at this wavelength, the amount of vitamin C released from the PCL can be quantified. A calibration curve can also be created by measuring the absorbance of known concentrations of vitamin C in buffer solution, allowing for more accurate quantification of the released vitamin C.

Other methods to quantify vitamin C release from PCL may include HPLC or titration techniques, but UV-Vis spectrophotometry is a relatively simple and commonly used method.

Dear Mr. Saman,

Thank you very much for your prompt response.

We have tried UV-Vis spectrophotometer and calibration curve but we are getting the release only after 1 hour time point and there is no further release after 1 hour !!!