Can anyone suggest ways to enhance the uptake of nanoparticles by cells under in vitro circumstances?
For example:
By surface modification or size alternation or other means? Please suggest whether there is some better option than these.
Dear Dr. Bloemen, thank you so much for sharing a very good literature. Thank you!
Hi, i agree with Maarten Sir. In addition, many times conferring nanoparticles with stealth property specific for the specific cell can also lead to enhancement of uptake by the cells. Also a higher degree of aggregation of nanoparticles has been found to decrease uptake by cells. Hence a decrease in aggregation can aid to enhance uptake of nanoparticle.
http://www.nature.com/srep/2013/130828/srep02534/full/srep02534.html
http://onlinelibrary.wiley.com/doi/10.1002/etc.2470/pdf
http://neilphilipodonnell.com/resources/images/neil_p_odonnell_thesis.pdf
If you are working with adherent cells, keep in mind that sedimentation will play a role in the kinetics of your nanoparticle uptake by cells.
Also, you can use a dedicated uptake buffer for the incubation, which you may allow to last up to 4 hours for most endocytic pathways to occurr.
Nanoparticle coating is quite simple to implement and can indeed yield satisfactory results ( but is doest
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