We need to know the level of sex hormones level after the menopause in mice. If anybody know the procedure for the same without using kit method (manually) then please let me know.
Thanks and Regards
You can not use kit method (manually) because there is just ELISA methods.
The effects of age, basal FSH and estradiol concentrations have been well documented (Scott et al., 1989; Scott and Hofmann, 1995; Toner et al., 1991b; Cahill et al., 1994; Licciardi et al., 1995; Mukherjee et al., 1996; Kim et al., 1997). However, the most important aspect of diminished ovarian reserve and associated decline in reproductive potential is that its onset is highly variable (Mosher and Pratt, 1990; 1991; Maroulis, 1991). This means that functional ovarian age as reflected by basal FSH and estradiol concentrations can be discordant with biological age (Scott et al., 1989; Scott and Hofmann, 1995; Toner et al., 1991b; Cahill et al., 1994). Patients who have normal FSH and estradiol levels or are younger than 35 years of age may still have a diminished ovarian reserve that leads to poor response to Gn stimulation (Farhi et al., 1997). They may have a decreased Day 3 serum inhibin B concentration despite the unchanged Day 3 serum FSH and estradiol concentrations (Seifer et al., 1997; 1999). Inhibin B was suggested as an early sensitive predictor of ovarian reserve (Seifer et al., 1997; 1999). With better understanding of the control of synthesis and secretion of the inhibins and their potential endocrine role in the menstrual cycle, attention has turned to the possibility of this family of peptides to provide a more direct index of ovarian reserve and an improved predictor of ART outcome (Hughes et al., 1990; Groome et al., 1994; 1996; Balasch et al., 1996; Lockwood et al., 1996; Seifer et al., 1997; 1999; Danforth et al., 1998; Corson et al., 1999; Hall et al., 1999; Peñarrubia et al., 2000; Laven and Fauser, 2004; Shanthi et al., 2004).
Your question is of interest, but needs to be more specific. If you mean monitoring their cycles manually, then using the historical vagianal swab method should give you a bit of information, but not absolute levels of hormones. If you see a consistent, static or constant pattern that continues for days, then this would indicate that the reproductive system is in a constant (post-menopausal) state. You could modify the states by removing one ovary or one ovary and part of the other ovary (surgically) so that menopause would occur earlier. Mouse lines also differ in reproductive life, so you might compare lines. If you are "creative" you might be able to produce new data by using historical vaginal swab approaches coupled with some creative approaches to alter number of follicles in the pool (by removing some ovary tissue from young mice). Good luck!
You can not use kit method (manually) because there is just ELISA methods.