Unhappy primary neuronal cell cultures during experiment?

Hi, I am currently running an experiment that involves a 10-minute incubation of live cultured neurons in PBS containing magnesium and calcium on ice. Following immunocytochemistry for the...

12 December 2020 1,210 3 View

Is there a tool for 3D miRNA modeling?

For example for an interaction study between a protein target.

22 November 2020 3,952 4 View

New network testing models for deep learning in biological testing ?

We tested four models: logistic regression (LogReg), multilayer perceptron (MLP), graph convolutional network (GCN), and edge-attention graph convolutional network (EAGCN). Are there other models...

08 November 2020 2,347 4 View

Where to find cancer datasets composed by clinical markers and gene expression levels?

Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...

11 October 2020 9,012 2 View

Can I use a Glass electrode (standard used for pH measurement) for measuring ORP?

I have made a test to investigated: I measured the potential in mV of a standard +220mV solution using my pHmeter which gives a mV value with the pH. The result was +9mV. But this was the result...

06 October 2020 7,220 3 View

How to do validation on a new instrument?

Halloo scientists, please help me the procedure s on doing validation on new bought instrument/equipment before starting doing analysis

07 September 2020 1,590 3 View

Can my RNA interference have any influence in my endougenos control gene?

Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...

25 August 2020 263 3 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

How do I stored nano particles in my workplace and how long?

I am looking for a technique which is used to store the nano particles without any property change.

23 June 2020 2,473 15 View

List of environmental journals with no publishing fees?

I'm looking for a well-indexed Environmental/ocean science journal to publish my research papers without any publication fee, could you help me?

20 May 2020 2,233 14 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View