How true is it that antibiotic coated/silicon catheters do not actually reduce the prevalence of catheter associated urinary tract infection?

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Is it possible for small molecule ligands to show significant heat release in control ITC run?

I am trying to optimize my control titration for ITC so that I can ensure my ligand to protein traces are reliable. My small molecule is stored in DMSO. I have been very careful to avoid buffer...

14 February 2024 9,147 3 View

Slight increase in heat transfer at the end of ITC trace?

Hello, I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat...

09 February 2024 4,680 1 View

Any tips on reverse ITC, protein into ligand?

Hello, I am having an ITC issue because I cant increase my ligand concentration due to solubility issues. I know there is past literature on reverse titrations being acceptable. I am more asking...

06 February 2024 5,564 2 View

How to elucidate molecular structure of unkwon? I have done FTIR, HNMR, CNMR & DEPTH 135. ?

Dear respected seniors, I'm a young ambitious researcher looking for guidance on how to elucidate molecular structure. I have done FTIR, HNMR, CNMR & DEPTH 135 analysis. The expected structure is...

28 January 2024 9,679 1 View

How to rule out buffer mismatch in ITC Curve?

Hello, I am doing ITC and getting repeatable curves that look very promising. After reading some articles, it seems the biggest challenge to avoid is buffer mismatch. Is there a way to rule out...

18 January 2024 1,224 5 View

ITC Software not fitting correctly?

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22 December 2023 9,331 1 View

ITC Curve in the opposite direction?

Hello, I am doing a titration of ligand into protein and am getting good heat releases compared to my ligand into buffer. I am getting more heat release in later injections. Could cooperativity...

07 November 2023 775 4 View

Hi i requested permission for a couple of articles from authors yesterday 13/10/23. How long does this usually take to get approval ?

I would like to know how long it takes to get access after requesting permission from authors for articles.

13 October 2023 530 2 View

Is there a dispersion relation for metal vapor density similar to plasma density?

Electromagnetic waves with frequencies approaching those of a plasma with a similar election plasma frequency, are highly reflected/absorbed by the plasma and can be predicted by a dispersion...

17 August 2023 2,737 0 View

Is there a way to know before hand a certain modification of an evolutionary algorithm will improve it's performance?

To start with, I want to believe for a modification of an algorithm like evolutionary algorithm to be considered novel, it has to offer some benefit (like automation) and should not perform worse...

14 August 2023 9,568 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

07 July 2024 8,671 6 View

How do you bleed HIV-infected humanized mice?

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27 June 2024 7,707 2 View

What are the best criteria for detecting Healthcare associated infections, especially in case of urinary tract infections?

This is an important step for isolation of hospital pathogens.

18 June 2024 5,633 1 View

What is the typical speed of steering a steerable catheter by a cardiovascular interventionist?

Let’s say a clinician is using a steerable catheter and we want to see the range of velocities the clinician manipulates the steering module with. Are there any references citing this?

17 June 2024 4,289 0 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

I want to ask if I suppose to add 200mg of antibiotic in 100ml of water so what is the concentration of this stock solution in microgram?

Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...

08 June 2024 2,285 6 View

Jonathan Betts

Several papers present research on this topic. Many state an antimicrobial coating, whether an antibiotic or metal, does not reduce the instances of CAUTI's

Some extra reading for you

http://www.ncbi.nlm.nih.gov/pubmed/23199586

http://www.ncbi.nlm.nih.gov/pubmed/23134837

http://www.ncbi.nlm.nih.gov/pubmed/25075308

http://www.aricjournal.com/content/3/1/23

http://www.journalslibrary.nihr.ac.uk/hta/volume-16/issue-47

Fouad Aoun

I agree however drainage in à close system reduce thé incidence of CAUTI