How TRPV4 Agonist GSK101stimulation should be given ?

18 August 2022 0 4K Report

I'm trying to push calcium inside cells by stimulating RAW macrophages with an agonist, but I didn't get any difference between GSK101 stimulated cells and unstimulated cells when I measured calcium fluctuation inside cells with the help of Fura 2 AM on plate reader.

I don't know what and where I'm doing wrong. I'm not able to figure out exactly what's happening.

I would appreciate it if the expert working in the said field could assist me with the most efficient way along with the protocols to assess the TRPV4 agonist-mediated calcium.

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