How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

What causes up shift of protein band in Western Blot?

Hello, I am working on a recombinant protein expressed in the form of inclusion bodies for my bachelor thesis. After solubilization and refolding I performed an SDS-PAGE and to confirm the...

21 October 2020 5,311 8 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Can you suggest an academic platform (in addition to RG and Academia) where PhD students can share drafts of their papers for feedback?

I am looking for a platform where PhD students would feel comfortable to share their papers at a very early stage of research and could receive a helpful feedback.

05 August 2020 4,713 3 View

Difficulty in interpreting data processed with two-way ANOVA?

I have a two-factor experiment. We investigated the effect of the drug on the level of erythrocytes after surgery. One of the factors is the presence of the operation, the second is the presence...

22 July 2020 7,687 5 View

Do you have information about the available free program for determining the area of leaves using a photo or image scanner?

21 June 2020 2,297 5 View

Please, help. Should one or two-way ANOVA be used in case of determining the drug effect of the drug against the background of the model operation?

We studied the effect of a biological supplement on biochemical and hematological parameters rabbits with and without model operation. I have 4 group of rabbits (n=8) and 3 time points (before...

15 May 2020 5,846 17 View

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

Does anyone know of a free biology software NOT SUBJECT TO COPYRIGHT for publishing papers?

24 February 2020 7,748 3 View

How to analyse measurements from 3 different conditions?

I have cytokine measurements from 3 different conditions: What is the best way of comparing the differences between the conditions ? Many thanks Irina

20 February 2020 5,750 3 View

Possible problems with eNOS intracellular staining in circulating endothelial cells?

I am trying to set up a protocol to study circulating endothelial cells by flow cytometry. First approach was to identify CD45-CD146+ population and analyze expression of cell adhesion molecules...

28 January 2020 9,190 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

How to do immunofluorescence staining of the microtome sections of paraffin-embedded tissue ?

I plan to do immunofluorescence staining of microtome sections of paraffin-embedded metastatic tumor tissue with two or more than two antibodies with different emission spectra. Should I follow...

10 February 2021 3,113 3 View

What can I do to keep magnetic beads suspended in solution within a syringe?

I'm looking for ways to prevent the settling of magnetic beads in solution. I have been using a syringe pump to inject a suspension of magnetic beads in PBS into a microfluidic device, and so far...

09 February 2021 6,206 2 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

30 January 2021 4,092 3 View

What is the Melting point criterion in a MD simulation?

I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.

26 January 2021 9,096 5 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View