What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Can someone recommend me a classic book of liquid crystals?

I am currently into liquid crystals and have no related background. Can someone tell me which book is the most instructive one to learn basics of liquid crystals? I am more interested in different...

22 November 2020 4,362 6 View

How does the influence of metal ions on the fluorescence intensity of a compound?

We found an interesting phenomenon that metal ions could not influence the fluorescence of a compound when the compound was dispersed in solvent. However, the fluorescence of the compound was...

31 October 2020 6,568 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Fluent how to draw a graph of coordinate changes over time?

When simulating waves, it is necessary to detect the coordinate change at a certain point of the wave surface, but there is no in fluent.

04 October 2020 1,541 1 View

How to quickly know the new research area landscape and find a research gap in this new area?

As a Ph.D. candidate who enters a new research area that differs from the research topic in the master period (e.g. shift form crop breeding to soil ecology), how to quickly know the new research...

23 September 2020 8,131 5 View

How to build epoxy model in GAFF for Lammps?

Dear all, I am a beginner in Molecular Dynamics. I build a pre-reaction epoxy model using Materials Studio and plan to perform the cross-linking procedure using Lammps. However, the msi2lmp tool...

26 August 2020 7,697 1 View

How to detect brown pigments generated in red wine aging process ?

During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...

17 July 2020 6,032 4 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

Which books and review articles describe well the current state of deep reinforcement learning for the optimal control of physical processes?

I am going to treat in a lecture the application of deep reinforcement learning for the optimal control of physical processes. Therefore, I am looking for the best references. Besides the classics...

16 February 2021 1,006 6 View

How to select the number of nearest neighbors in relief algorithm for feature selection?

For feature selection of a binary class dataset, relief algorithm chooses an observation randomly and then selects nearest neighbors of that observation from both classes. Selecting number of...

09 February 2021 4,991 2 View

What are the engineering methods for feature selection in machine learning?

Hello researchers, I have a dataset that contains 36733 instances, What are the engineering methods to be used for feature selection in machine learning?

09 February 2021 363 11 View

Why we use feature normalization before using the Gaussian kernel in SVM?

Support vector machine

09 February 2021 6,405 3 View

How to know the threshold limit value of a sar image...??

i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.

26 January 2021 8,277 1 View