I want to add additional control for STZ induced diabetic mice. I have one vehicle injected control. Can anyone help me how to generate STZ- insulin control? I would like to know how much insulin dosage should be used and the timeline?
Should mice be fasted before STZ injection for induction of diabetes?
01 February 2017 2,253 5 View
Can anyone suggest which glucometer is good for testing hyperglycemia in mice? I have to check hyperglycemia in STZ injected mice
10 November 2016 404 5 View
can we use Neurobasal A media 5 months after expiry which is new unopened?
02 March 2016 4,792 0 View
can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane? Is it reversible?
02 March 2016 4,379 6 View
how do i prepare the positive control from a purified peptide for western blot?
11 December 2015 2,634 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
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05 August 2024 4,407 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...
03 August 2024 5,358 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View