How to use bio-rad gene pulser cuvette?

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Which journal is suitable to publish ISI-low impact factor for protein modeling/bioinformatics/structural biology?

I want to publish manuscript related to protein modeling with low impact factor,indexed by ISI, scopus..

01 February 2015 8,166 6 View

How can I predict and evaluate this DOPE profile?

Can anyone give comments for this DOPE profile? Thank you.

31 December 2014 5,580 1 View

How to download this article? thanks.

Floral volatiles from biosynthesis to function

11 December 2014 7,538 0 View

Hi,what is the suitable modelling server for the size of amino acid sequences, ranging from 300 to 450 amino acid residues?

I am a novice user for using protein bioinformatics tools. I don't know the clear path for analyzing my amino acid sequences of interest for modelling 3-D structure. What is the initial step of...

11 December 2014 8,654 3 View

How to open Geno3D?

I cant open it after clicking its web link on google.

11 December 2014 6,963 0 View

How can one use TSP-PRED properly for submitting protein sequences?

I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.

11 December 2014 3,233 6 View

How to change to .ali file and .py file?

My current files for modeller 9.14 (Mod9.14) were all in notepad, except for protein sequences in pdb format. I dont know how to change my files to .ali and .py format. Any suggestion?

11 December 2014 9,613 2 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

07 August 2013 6,039 12 View

Separating gel does not solidify. How to troubleshoot?

Is it necessary to put any liquid on top of TEMED-treated separating gel mixture between spacer plates with 1.0 mm Integrated spacers in closed casting frame?If yes, what type of liquid...

06 July 2013 305 46 View

Heartburn. Is it an early warning sign of cancers?

Is it true that the use of H2 blocker, proton pump inhibitors and other antacids can lead to the progression of recurring heartburn? The effect of reducing stomach acid can also make gastrin to be...

06 July 2013 2,143 5 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Bony De kumar

0.4cm gap width cuvvettes are used for mammalian cells or ES cell electroporation as per bio-rad recommendation.

Ahmed Mohamed Abdelbaki

did it work with with you ?

Winschau Fayghan Van Zyl

0.4 cm gap size cuvette works with E. coli.

Jianghai Liu

Hi Winschau, I am interested to the transformation efficiency when the 0.4cm cuvette was used for E. Coli. Thank you! I want to try it too.