How to use algae extract to germinate rice seeds?

Fateme Khajepour @Fateme-Khajepour

20 July 2023 1 7K Report

Green algae or blue green algae?

Mohd Ariff Ahmad Nazri

Materials Needed:

Algae (green algae or blue-green algae) culture or extract

Rice seeds

Sterile water (distilled or deionized)

Spray bottle or dropper

Petri dishes or small containers

Plastic wrap or lids (to cover the containers)

Procedure:

Prepare Algae Extract: If you can access live algae culture, you can prepare the algae extract by blending the algae with sterile water and filtering the mixture to obtain the liquid extract. Alternatively, you can use that directly if you have access to pre-prepared algae extract.

Sterilize Rice Seeds: Soak the rice seeds in a 10% bleach solution for about 5 minutes to sterilize them and minimize the risk of contamination.

Rinse Seeds: After sterilizing, rinse the rice seeds thoroughly with sterile water to remove any remaining bleach residue.

Germination Setup: Place the sterilized rice seeds on a moistened paper towel or filter paper inside a petri dish or small container. Ensure the paper is sufficiently moist but not soaking wet.

Add Algae Extract: Using a spray bottle or dropper, apply the algae extract to the surface of the moistened paper towel, ensuring the extract is evenly distributed over the seeds.

Cover and Seal: Cover the petri dish or container with plastic wrap or a lid to maintain a humid environment for germination.

Germination Conditions: Place the covered petri dishes or containers in a warm and well-lit area. Rice seeds germinate best at temperatures between 25°C to 35°C (77°F to 95°F).

Monitor Germination: Check the seeds daily for germination progress. You should start to see the first signs of germination within a few days.

Algae Type: Both green and blue-green algae can potentially be used to create an extract for germinating rice seeds. Both types of algae contain beneficial nutrients and growth-promoting substances that can support seed germination and early seedling growth. The choice between green algae and blue-green algae may depend on the availability of the algae culture or extract and any specific properties you may want to explore. Green algae are typically easier to find and culture, while blue-green algae (cyanobacteria) are known for fixing atmospheric nitrogen, which can benefit plant growth.

It's worth noting that the success of using algae extract for seed germination may vary depending on factors such as the quality of the algae extract, seed variety, and environmental conditions. Experimentation and observation will help you determine the effectiveness of algae extract for germinating rice seeds in your specific situation.

Badges
Science topic

More Fateme Khajepour's questions See All

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

How to calculate DNA concentration of a sample with high concentration buffer?

I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...

23 January 2021 9,440 2 View

Can DNase1 degrade the plasmid (circular) DNA?

I had a sample of plasmid extracted from e.coli and wanted to check the amount of Host cell RNA impurity with a fluorescent dye. to do this, first of all, I treated the sample with DNase I. (NEB...

11 January 2021 2,608 3 View

VASP - Convergence Problem?

Dear All, I am new to DFT calculation, and I am using VASP. In a lot of my jobs, the forces are already smaller than the EDIFFG tag that I set in the INCAR, but, the jobs won't stop!!! The jobs...

26 December 2020 1,045 6 View

Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

Where can I find a Twitter dataset for Preliminary Flu Outbreak Prediction Using Twitter Posts Classification?

07 December 2020 4,700 2 View

How to generate DRR (digitally reconstructed radiography)?

I am studying 2D and 3D registrations. I need help and guidance in producing DRR from CT images. Is there an article or program that fully explains how to generate DRR from CT images? . I want to...

22 October 2020 7,876 4 View

What are measurement criteria of bacteriophages in TEM pictures?

I've got the TEM pictures of my purified bacteriophage whose host is S. aureus. there are various shapes with tail/tailless (maybe a little tail). in order to place it in a family, I need the...

15 October 2020 5,261 5 View

What is the reasons of appearance exothermic peak and oxidation in DTA results of HEAs?

Hi I synthesized a CoCrFeMoMnNi High Entropy Alloy by the VAR method. After DTA analysis, the sample was completely oxidized and an exothermal peak was observed. Due to that, the structure is FCC...

18 August 2020 7,204 3 View

What Can I do if I have not normality in my large scale sample(1122)?

I have 1184 sample for my test. They are in 5 age group. all of my data does not have normality and variance of my groups are not equal. what I can I do? Can I use parametric tests?

07 June 2020 422 11 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

What are the new researchable area under Rice crop under Agronomy related to moisture stress and nanotechnology ?

I had already worked on the moisture stress and lot of works being done in rice regarding stress or drought. I want to know some very recent researchable areas in rice crop regarding moisture...

26 January 2021 5,518 7 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Is it possible to separate the momilactone molecule by GPC device?

The momilactone molecule is present in rice and has an allochemical effect on weeds.

01 January 2021 3,367 3 View

How can I use amylase enzyme to digest molluscan tissue before DNA extration?

Hi, I have a problem with a hight contet of mucopolysaccharides in some molluscan samples. I tried different DNA extration procolols but I can't already remove all the molecules that inhibit PCR...

01 January 2021 8,900 3 View