For my independent research project for a microbiology lab, I am working with isolated Staphylococcus and P. acnes against 6 over the counter acne medications. I've done a modified Kirby-Bauerr method in testing for antimicrobial sensitivity and got great results. I'm supposed to perform a different procedure that tests for sensitivity.
I want to do a modified MIC method but I am having trouble in the serial dilutions of the creams.
Is there a way to create a suspension of the creams so that I can perform the serial dilutions? If I combined sterile saline with the cream, suspended it, would this disrupt the chemical compounds or would this be acceptable? I would then have to dilute that suspension to get different concentrations, correct?
Or is there a way to plate different concentrations of the creams and then plate the organisms onto it?
This is with undergraduate university lab equipment. We have spectrometers, plates, tubes, etc, but nothing advanced.
Thank you!
Hi,
When you say your test sample is a cream, would it be soluble in saline? Another point that I would like to add is always have a control for the diluent material. You will understand if it has a minimalistic background activity too. You could actually perform serial dilutions.
I performed the serial dilutions! I created suspensions of the creams in saline, which created a cloudy, semi-white solution. I created different concentrations of the creams and then added the bacteria for it to be plated. Thanks for the response!
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