I am going to treat the myocardial infarction rats with spironolactone for 8 weeks/56 days. As you know, there are several ways to administer this drug, such as oral (gavage and mixed in rat chow), intraperitoneal and subcutaneous injection. We currently cannot mix it with rat chow.
Which technique do you think is more prior?
How to make the solution of it? Can we make the solution with physiological serum?
Your advice and suggestions will be much appreciated and welcomed.
Spironolactone acts by antagonism of aldosterone in the distal renal tubules. Inhibit cardiac collagen proliferation after myocardial infarction in rats and fibrosis of dilated atria , Spironolactone administered at dose (20 mg/kg per day) for 1 month .
References :
Yu HD 1 ,Xia S , Zha CQ ,Deng SB , Du JL , She Q .Spironolactone Regulates HCN Protein Expression Through Micro-RNA-1 in Rats With Myocardial Infarction. Journal of Cardiovascular Pharmacology [01 Jun 2015, 65(6):587-592].
Mill JG1, Milanez Mda C, de Resende MM, Gomes Mda G, Leite CM.
Spironolactone prevents cardiac collagen proliferation after myocardial infarction in rats. Clin Exp Pharmacol Physiol. 2003 Oct;30(10):739-44.
You can use ip and sc injection. to my experience sc is the best for long lasting treatments. you could use DMSO, corn oil or sesame oil as vehicle. gavage is not very suitable, because not only it's stressful, but also can seriously damage the esophageal and gastric tract.
Check this link
https://www.ncbi.nlm.nih.gov/m/pubmed/26065643/
regards
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