How to TLC of hydrophilic compounds?

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Kimberly Delica

How hydrophilic compounds is your sample? Try using 2% acetic acid in methanol or reverse-phase TLC. Perhaps you can directly optimize in HPLC-reverse phase

Rajarshi Rit

@kimberly Delica can it be done on ordinary TLC plates? Reverse phase TLC plates are very costly

It really depends on how hydrophilic your sample is. If you use the normal phase TLC, try optimizing your solvent system with 2% acetic acid in MeOH or you can go up until 5% acetic acid. Just make sure, the silica on TLC wont be dissolved.

Chen Jingwen

Chromatography polyamides can be used to separate water-soluble components.