Dear researchers,
i want to test for statistical differences between different sample sites. I´ve sequenced microbiome of 16 biogas plants and 10 sewage treatment plants, and get an otu table, which comprise OTU´s absolute abundancies for each plant.
Now i would like to test whether the OTU composition is significantly different:
A) between the two groups of plants
B) between each plant
For A i would do ANOSIM and for B i would prefer an one way Anova.
Is that acceptable or are there any other suggestions?
Should i do the analysis with the relative or absolute abundance. Is it necessary to transform data before analysis?
Thanks for your help, as you might already know, i´m no statistic crack.
Thank you very much,
Christian
Why don't you undertake photogenic studies to see 16 sequenced microbiome are different to each other or same or else how many minimum genus they represent using similarity index...
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