As I read through, the this resistance gene can be synthetically produces. But I cannot grab the protocol. Can anyone describe to me on how to synthetically production of Chloramphenicol resistance gene cassette?
What are the most effective remote sensing techniques and algorithms for detecting and analyzing land use and land cover (LULC) changes in rapidly urbanizing areas, and how do their accuracies and...
11 July 2024 6,695 1 View
My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill
09 July 2024 9,078 4 View
I have tried to resolve this issue a little bit, but eager to get your insights and thoughts to make my efforts better. Using Homophones and Homographs in ESL Classrooms
04 July 2024 6,742 3 View
The contribution of "Distance Education" is immense, but still needs a lot of reforms. Universities generally adopt one another ideas and ignore the ideas of world wide scholars, distance...
04 July 2024 2,545 6 View
"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...
01 July 2024 8,406 5 View
In educational institutions where English is taught as a second language, only 25% of listening happens in English but rather 75% of listening takes place in other language like Hindi/Urdu/Punjabi...
28 June 2024 6,353 4 View
How do we compare the Rf values of the spots to known standards or literature values to help identify the compounds. How TLC does allows the identification and analysis of the mixture of...
07 June 2024 1,967 5 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
Theoretically, at lower A/C excitation frequencies; say around 100 Hz, penetration of 5-10 mm is achievable in carbon steel (ferromagnetic) material. But, there is literature suggesting that this...
10 March 2024 548 0 View
I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...
28 February 2024 213 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...
02 July 2024 8,480 0 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
thanks
25 June 2024 4,667 0 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
23 June 2024 6,583 2 View