How to submit ITS-rDNA partial gene in Bankit?

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Why should you convert a WGS to core genome? and what is a core genome?

I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.

10 June 2024 8,347 1 View

Who can help in astrophysics modelling?

I need some one to help in modelling an astrophysics hypothesis. Call WahtsApp 00962777526193 email: [email protected]

15 May 2024 6,043 1 View

Is there alternative to plausibility test (sensibility of strings) if native English speakers are not available due to non-native context of research?

Stimuli norming prerequisite of plausibility test is the issue in nonnative context of research. Regards

19 March 2024 8,471 0 View

How can you upload more than 1 sequence at Resfinder?

I learnt to add 1 sequence at once but couldn't find an option to upload a bunch at once, still a tyro at bioinformatics, any leads?

27 February 2024 7,514 1 View

Please guide under what synthesis conditions I can control/eliminate the amount of organic carbon in the soot??

Synthesis of organic carbon free soot

28 January 2024 9,124 5 View

I want to measure band gap of soot using Tauc plot method. Please guide,which solvent can be used to extract both organic carbon and elemental carbon?

Solvent for the extraction of OC and EC from Soot

23 January 2024 9,446 3 View

How to convert International unit (IU) to mg/ml if specific activity is not given?

I have to find the concentration of 3 million IU of interferon-alpha in mg/mL or micromolar.

28 December 2023 8,460 0 View

What recommendations are required for process enhancement for sand casting methods?

Aircraft engine parts are usually manufactured from high precision casting methods such as investment casting. This is because the quality is utmost important for such applications as human life...

17 December 2023 8,780 0 View

How can I make my nanoparticle size smaller?

Hello! I'm a 1st year Masters student working with nanoparticles. I've been trying to formulate nanoparticles for awhile now and I've been struggling to get my particles to its true nano-size...

05 December 2023 8,964 3 View

How can we design primers to check mutation in genes, but not expression of genes through RT-PCR analysis?

I have designed primers to see the gene expression of some targets, but now I want to check the mutation of those genes through PCR. How can I design primers for the mutation of genes?

04 October 2023 8,185 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View