How to submit complete plastid genome sequence to genbank?

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How do you obtain a Letter of Access for Citizen Researchers?

Please may I ask if non-salaried Public Contributors conduct data collection interviews or Focus Groups with NHS patients as part of your study? This means that the Public Contributor will either...

29 July 2024 3,085 0 View

Can DEPC-treated water be used as a replacement for OCT when embedding frozen tissue on cryostat?

Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...

25 June 2024 7,550 1 View

How to write literature review?

i want to write literature review on the topic " production of 3d PLA filament"

19 June 2024 1,712 2 View

Does Trizol LS yield more and better RNA than normal Trizol?

Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...

18 June 2024 6,854 3 View

Unstructured Interview Design?

In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...

15 June 2024 3,884 1 View

What could dissolve MgO nanoparticles over time?

I have a set-up were I have a bottle with water (DI water, 0.8uS/cm). To that bottle I have added some MgO nanopowder, at a concentration of ~ 0.08mg/ml. It appears that the white powder is...

04 June 2024 9,789 4 View

What strategies are being employed to mitigate the environmental impacts of urbanization, particularly regarding habitat loss and fragmentation?

As urbanization accelerates, the environmental toll of rapid urban growth becomes increasingly apparent. Please pitch your opinion/idea on this question as we explore innovative approaches...

02 June 2024 737 3 View

Histology - mitochondria: Hi RG members. Is anybody aware whether a reliable method for determining mitochondrial density exists for light microscopy?

I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...

02 June 2024 5,373 2 View

Is my researchGate profile going to be visible on search engine ?

My profile on researchGate is not yet visible on goggle

26 May 2024 5,824 1 View

How do climate change-induced alterations in precipitation patterns impact freshwater availability and quality in different regions globally?

Climate change-induced shifts in precipitation patterns are reshaping the availability and quality of freshwater resources worldwide. Join us in examining the far-reaching implications of these...

19 May 2024 8,764 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View