How to study the intercalation between organic molecules and DNA if the molecule is not soluble in th buffer?

14 December 2017 1 1K Report

I did my experiments (UV vis for determination of Kb) adding a solution of my compound in acetonitrile into the aquous media where DNA stands. I don't know if this is correct, cause the organic solvent can make DNA precipitate or something like that. What do you think about it?

Adam B Shapiro

Find out whether the compound is fluorescent. If it is, then you can potentially use a much lower concentration to measure binding to DNA than is required for absorbance measurements, and the compound may be soluble at the lower concentration.

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Science method

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