How to study a complete profile of miRNA/small RNA level in polymorphonuclear (PMC) cells from blood isolate?

More Kathirvel Maruthai's questions See All

What kind of cell line will be most suitable to study TB infection and gene expression in a host?

I am planning to study the epigenetics of TB infection in the host system. I have to quantitatively analyze the expression pattern of certain genes viz., IFN-g, TNF-a, TLR-4, FOXP3...etc. and...

08 September 2014 6,141 13 View

Can someone advise on the genetic analysis of Treg and Tef cells?

I want to study the transcriptional changes in T cells in the context with TB infection. I have a doubt as whether flow cytometry or Flowcytometry with cell sorter will be the best one to do.

09 October 2013 2,302 2 View

How to find the primer binding sequence of a target gene?

I have a handful of primers, of which the details are unknown. In particular, I want to find the exact target sequence bases of the primers in their respective genes. So, I tried to find out, but...

11 December 2012 4,695 7 View

Lack of PCR product visibility in gel?

I ran PCR for the detection of M.tb infection in clinical specimens. Sometimes I get the PCR product as a faint line while running the gel. Could anyone tell me how could I resolve this?

11 December 2012 9,712 8 View

Will proteins get denatured in Ammonium Sulphate precipitation?

While doing extraction of proteins by using ammonium sulphate as salt system, and if it is left to be in salt does it affect the protein nature such as denature, activity lose, changes in...

08 September 2012 2,691 13 View

Difficulties in RNA isolation?

I am going to start my protocols in RNA isolation, both qualitative and quantitative. What difficulties should I have in my mind? and what will be the solution for that?

08 September 2012 998 7 View

Gel Image banding pattern analysis by Dendrogram?

Could anyone tell me how to put dendrogram based on the gel image? I have attached my gel image as sample. What are the possibilities to proceed with?

07 August 2012 1,734 12 View

How to inactivate the action of 5-azacytidine in cell culture to study further DNA methylation effect specifically after infection in same culture?

At first, I am planning to de-methylate (existing methylation) my culture with 5-azaycytidine drug and want to inactivate the further action of this drug. Then I am planning to infect my culture...

01 January 1970 5,680 1 View

How to compare 2 samples recruited with different sampling methods?

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26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

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15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

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04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

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Nicolas Luigi Pascal Casadei

You want to make a Chip analysis? Like Affymetrix?

On this case the best will be to contact work group or company specialized on it.

Or you want to go for sequencing?

Kathirvel Maruthai

Thanks for your reply. Yeah i will have look about both the things. and one more clarification as if I go for sequencing means what will be efficiency between the chip analysis?

Sorry I do not get the question.

I guess this paper should already solve a lot of your questions

1: Pritchard CC, Cheng HH, Tewari M. MicroRNA profiling: approaches and

considerations. Nat Rev Genet. 2012 Apr 18;13(5):358-69. doi: 10.1038/nrg3198.

Review. PubMed PMID: 22510765.

Best regards

Thanks. it's my pleasure to see this.