I have tried to attach cells to the PDMS wall of a microfluidic device but my cells are loosely attached and wash away after exposing them to flow (low flow rates, about 0.0004mL/hour). by incubating with 0.2% gelatin for two hours and then subsequently washing with PDMS +/+ and then adding the cells. I can see that the seeding ratio is good, and then I will incubate for about 45 minutes before adding media. Is there a way to more firmly adhere the cells?

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