Joshua Hall

6 Questions 2 Answers 0 Followers

Questions related from Joshua Hall

Public Datasets with Pre-Processed Invasive XA data for lumen segmentation training?

I have found the dataset DCA1 that meets this requirement, but need additional data that is publicly available (or if anyone knows collaborators who would be willing to share. Does anyone know if...

04 April 2023 3,199 0 View

Can I examine chemokines using a western blot from total endothelial cell lysate?

After qPCR analysis of HUVEC lysate from my experiment, I found upregulation of several chemokine mRNA that I would like to validate via western blot. Due to the nature of my experiment, it would...

16 December 2019 5,524 3 View

Why does my HEPES pH changes from 6.9 to 7.5 in 24 hours?

I am adding 25mM HEPES buffer to EGM2 media to culture HUVECs. I initially buffer the solution to 6.9 (I started at 7.1 but I lowered it a bit to compensate for the pH rising), but in just 24...

21 May 2019 2,482 3 View

How to strongly adhere endothelial cells inside PDMS device?

I have tried to attach cells to the PDMS wall of a microfluidic device but my cells are loosely attached and wash away after exposing them to flow (low flow rates, about 0.0004mL/hour). by...

04 March 2019 9,701 1 View

Difficulty cryosectioning lymph nodes and small intestine?

I am trying to cut 20 um cross sections of murine lymph nodes and small intestines, however, the tissue is fatty and not freezing thoroughly. When I cut the OCT, the cut will have a hole in the...

09 October 2018 333 1 View

How to remove silicon wafer shards from PDMS?

After removing my PDMS device from my silicon master, it shattered and left silicon fragments within the features of the device. I have tried using scotch tape to remove them and also washed it in...

05 September 2018 6,536 1 View