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After qPCR analysis of HUVEC lysate from my experiment, I found upregulation of several chemokine mRNA that I would like to validate via western blot. Due to the nature of my experiment, it would...
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I am adding 25mM HEPES buffer to EGM2 media to culture HUVECs. I initially buffer the solution to 6.9 (I started at 7.1 but I lowered it a bit to compensate for the pH rising), but in just 24...
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I am trying to cut 20 um cross sections of murine lymph nodes and small intestines, however, the tissue is fatty and not freezing thoroughly. When I cut the OCT, the cut will have a hole in the...
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