Hello, do you have any advice on how to store a purified viral protein for a long time? I have already noticed that after twenty days at 4 degrees it starts to form aggregates and precipitate.
I have thought about aliquoting it and putting it at -80 degrees, however I don't know if I should add something to avoid damaging the protein during freezing and thawing.
I use this protein for X-ray crystallography, plus I'm studying the interaction with a ligand, so I don't want to add glycerol as it could get in the binding site.
Do you have any advice?
Dear Luca
it depends from the protein properties.
Storage at -20°C for some proteins is ok even with out addition of any crioprotectants why in some cases addition of glycerol 15% could be usefull to protect it from aggregation.
iyou can see an example of it at the minute 3 of the following presentation avialable on my blog, ProteoCool:
https://www.blogger.com/video.g?token=AD6v5dzr9KqZslTx9NeEjXx1XyPti8dU15eqYoE9TT8OEDaZQcNINuDKwzCAzVXkFWZA0btipKTr81doABh-VjpprwCcRukVcLhAXE_T5BzPxYbeQQA7VMFmc4DdpjS4XbOfxkwAhIWu
best
Manuele
Now, the question at this point may be, how can I tell if my protein has a problem after freezing? A DLS experiment?
In any case I tried to aliquote the protein and freeze the tube in liquid nitrogen and keep the tubes at -80 C.
Thank you for your reply and interesting presentation.
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