Pisapia Luca

5 Questions 4 Answers 0 Followers

Questions related from Pisapia Luca

Which biophysical technique would you use to evaluate a low-affinity interaction in the mM range?

Hi everyone, I am working on a viral protein and its interaction with sugars. From the literature, it seems that the interaction is in the mM range (around 20 mM). I am using ITC, but...

16 July 2025 102 3 View

5nm gold nanoparticle detection by X-ray crystallography?

Hello, I was wondering if it is possible to detect a modified nanoparticle (5nm diameter) in the binding site of my protein using X-ray crystallography. The protein I want to co-crystallise with...

29 April 2025 3,509 1 View

Protein crystal soaking to have the ligand into the binding site?

Is it a good idea to try to achieve binding of my protein to a ligand by soaking, even though the Kd of the interaction is in the mM range? I have many drops with the crystallised apo-protein from...

05 February 2025 7,462 4 View

How to store a purified protein for a long time?

Hello, do you have any advice on how to store a purified viral protein for a long time? I have already noticed that after twenty days at 4 degrees it starts to form aggregates and precipitate. I...

16 December 2024 8,524 2 View

Why do my protein crystals form clusters?

I am trying to cocrystalize my protein with a sugar ligand, for this protein in particular I obtain only clusters, I tried to play around the pH( from 4,5 to 8,5) using the condition with clusters...

12 November 2024 9,145 2 View