I try to stain the Acetylcholinesterase and the Cholin-acetyltransferase in neuromuscular junction of diaphragm of mice with ALS model. I use abcam antybodies - Anti-Acetylcholinesterase antibody [HR2] ab2803 and Anti-Choline Acetyltransferase ab34419, I use standart IF protocol.
How to stain these enzymes in NMJ for IF method? Has anybody stained it?
You may find the method used by Liu et al. useful.
Article C9orf72 BAC Mouse Model with Motor Deficits and Neurodegener...
You may want to consider using fluorescent dye-conjugated fasciculin 2 to label the acetylcholinesterase, a method first utilized by Ben Peng, Richard Rotundo and their colleagues (Article Acetylcholinesterase Clustering at the Neuromuscular Junctio...
). See also (Article The Use of Synaptic Basal Lamina and Its Components to Ident...
). Hope this helps.- Badges
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