I'm directing some end-of-degree work in pharmacy. It is a question of mobilizing by the electrokinetic process of some dyes. The experiment in its development is simple except in the "analytical part".

Once I have fragmented the contents of the cell into 5 discs, I suspend them in water in a proportion of 10 grams of soil to 100 grams of water. I shake it for a day or two and centrifuge it, filter it through normal filter paper, then re-centrifuge it and then filter it again with ultrafine filter paper.

After all this it would seem easy to use visible-ultraviolet spectroscopy to determine the absolute or relative amount of dye mobilized. The "problem" is that the dissolution is still slightly cloudy (the soil scientists tell me because of the amorphous clays contained in the soil) What do you think I can do to improve my laboratory practice and not make it too complicated for the students?

Thank you very much and I look forward to hearing from you.

Felipe Hernández Luis

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