How to solidify a natural dye for isotherm analysis ?

More Yasashri Ranganath's questions See All

How does rhodamine dyes show signals in EPR ?

Hi yall, We are currently working on rhodamine involved research project. Does anyone know how rhodamine molecules give EPR signals? I mean the mechanism behind the unpaired electron formation of...

12 October 2020 5,512 2 View

Need guidance , (or) suggestin (or) reference materail for selceting 3dprintable bone material?

Hi Greeting to all I am mechanical Engineering doing research in 3d printable bone. ( my problem statement is I want to print a bone and to insert in the human body to find the deviations as a...

18 June 2020 2,406 9 View

Does anyone has a practical procedures for different voltammetric applications?

I want to try several well established voltammetric practicals up-to now, for that case i want to find practical procedures of different voltammetric applications. If someone can suggest...

15 January 2019 5,246 3 View

What is the reliability of a data in BET study for Nitrogen adsorption?

We got 0.2 m2/g-1 as the pore size in a BET study for Nitrogen adsorption. Is this data is reliable enough for include in a research paper or are there any conclusions can be made why we got this...

03 November 2018 7,687 11 View

Can anyone provide the link or reference of permissible limit or standard value of heavy metals in chicken and beef liver (According to WHO)?

I need details information about permissible limit of Pb, Cd, As, and Zn in chicken and beef liver

07 July 2017 6,190 4 View

How to remove hexane from a extracted essential oil sample ?

There is small quantity of extracted essential oil using hexane as the solvent, and the extracted sample contain oil and the hexane, it's a 5 mL sample. Is there a method to remove hexane and get...

18 March 2017 3,514 7 View

RNA extraction from encapsulated cells troubleshooting

I am encapsulating human mesenchymal stem cells in 10wt% polyethylene glycol diacrylate hydrogels. I have to extract total RNA from these cells. So the first step is homogenizing these gels to get...

10 February 2013 5,338 8 View

Could any one explain why a rotavirus adapted to MA-104 reduced its titre or disappeared from the 2nd passage onwards?

I am trying to adopt a rotavirus from clinical samples to MA-104 cell line, the virus starts producing the CPE from the second passage and virus presence was confirmed by ELISA and RT-PCR from...

20 May 2012 5,986 3 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Leo Vleugels

Hi Yasashri,

Natural dyes are generally not very stable, so heating or extreme pH-values are out of the question. I have had success with freeze-drying samples or natural dyes. Once you cleaned up the extract (removed solids and maybe further purified it), quickly freeze it in liquid nitrogen and next freeze-dry it gently, overnight.

In this article they recommend freeze drying as well: https://www.researchgate.net/profile/Ramamoorthy_Siva/publication/228365046_Status_of_natural_dyes_and_dye-yielding_plants_in_India/links/0c960528f20dd64f91000000.pdf

I hope this helps, kind regards, Leo

Article Status of natural dyes and dye-yielding plants in India

Yasashri Ranganath

Thank you very much Leo Vleugels,

I'll try in that way....